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KMID : 0903520050480020183
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 2 p.183 ~ p.188
Use of Spectrophotometry for Quantitative Determination of Soil Clay Content
Park Soon-Nam

Kim Kye-Hun
Kang Ji-Young
Abstract
This study was conducted to develop a method for the quantitative determination of soil clay content by spectophotometry. The optimum wavelength obtained with reference clay minerals for spectrophotometry was 500 nm. For the proposed spectrophotometry, 0.5 g of soil sample was put in the 250 ml Erlenmeyer flask and 100 ml dispersing agent was added. After shaking the flask at 130 rpm with a mechanical shaker overnight, the flask was removed from the shaker and was shaken up-and-down for 30 seconds. With a micro-pipet, 4 ml of the suspension was transferred into the previously-inserted cell and the absorbance was measured instantly. Results by the spectrophotometry for clay content analysis were compared with those by the conventional sedimentation technique (the pipet method). The proposed equation was y = 38.03x1 ? 0.17x2 ? 1.17, where y, x1, and x2 were clay content (%) by the pipet method, water content corrected clay content (%) by spectrophotometry, and organic matter content (g¡¤kg?1), respectively. The regression coefficient for the equation was r = 0.984**, indicating highly significant correlation between the results of the two methods.
KEYWORD
spectrophotometry, pipet method, clay content, particle size analysis
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